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Nonprofit · EIN 99-2011215

TWO FRONTIERS PROJECT INC

Tax-exempt · FORT COLLINS, CO 80526

Financials

$538K$256K2024
Revenue Expenses · by fiscal year end
Financials of TWO FRONTIERS PROJECT INC by year, from its e-filed returns
Year endedFormRevenueExpensesNet incomeTotal assetsLiabilitiesNet assets
December 31, 2024990$538,377$255,772$282,605$285,745$3,390$282,355
Financial details: 990 for the year ended December 31, 2024

Revenue

Contributions, gifts and grants
$538,219
Program service revenue
$0
Investment income
$0
Other revenue
$158

Expenses

Grants and similar amounts paid
$50,000
Compensation of officers, directors and key employees
$12,174
Salaries, compensation and benefits (total)
$12,915
Professional fundraising fees
$0
Legal fees
$550
Accounting fees
$7,340
Travel
$33,596
Depreciation
$2,300
Program services (functional)
$232,576
Management and general (functional)
$21,573
Fundraising (functional)
$1,623

Balance sheet and other

Total assets, start of year
$0
Total assets, end of year
$285,745
Total liabilities, end of year
$3,390
Net assets, end of year
$282,355
Land, buildings and equipment
$20,700
Net assets without donor restrictions
$282,355
Gross receipts
$538,377
Employees
1
Volunteers
4
Year formed
2024
State of legal domicile
DE

From the e-filed returns the IRS publishes as XML (latest three processing years; amended returns replace the original). PDF copies at the IRS.

Officers, directors and key employees

As listed on the 990 for the year ended December 31, 2024, highest compensation first.

NameTitleHours / weekCompensationOther / benefits
KRISTA RYONTREASURER AND DIRECTOR OF OPERATIONS
Trustee or director, Officer
40$4,263$1,507
BRADEN TIERNEYPRESIDENT AND EXECUTIVE DIRECTOR
Trustee or director, Officer
40$0$1,510
JAMES HENRIKSENSECRETARY AND DIRECTOR OF RESEARCH AND DEVELOPMENT
Trustee or director, Officer
20$0$0
ERIC WARDDIRECTOR
Trustee or director
2$0$0

Address and contacts

Address (IRS master file)
3336 DUDLEY WAY, FORT COLLINS, CO 80526-2573
In care of
% BRADEN TIERNEY
Principal officer (990 for 2024)
BRADEN TIERNEY, 3336 DUDLEY WAY, FORT COLLINS, CO 80526
Signed the 990
BRADEN TIERNEY, EXECUTIVE DIRECTOR
Phone
(781) 626-2626
Return prepared by
WEGNER CPAS LLP

Mission

THE PRINCIPAL PURPOSE OF TWO FRONTIERS IS TO ADVANCE HUMANITY'S UNDERSTANDING OF LIFE'S ADAPTATION TO EXTREME ENVIRONMENTS THROUGH SCIENTIFIC RESEARCH AND EDUCATION. THE ORGANIZATION PLANS AND LEADS SCIENTIFIC EXPEDITIONS TO REMOTE OCEANIC, TERRESTRIAL, AND OTHER HIGH-STRESS ENVIRONMENTS TO STUDY HOW MICROORGANISMS SURVIVE AND INFLUENCE NATURAL SYSTEMS.USING MODERN SEQUENCING AND MICROBIOLOGY TOOLS, TWO FRONTIERS COLLECTS, ANALYZES, AND SHARES DATA TO SUPPORT ENVIRONMENTAL RECOVERY AND CONTAMINATION MITIGATION. ITS OPEN-ACCESS DATABASES, SOFTWARE, AND PUBLICATIONS ARE FREELY AVAILABLE TO RESEARCHERS FOR NON-COMMERCIAL PURPOSES. THE ORGANIZATION MAY ALSO FUND ACADEMIC OR NONPROFIT PARTNERS TO CONDUCT RELATED SCIENTIFIC STUDIES THAT CONTRIBUTE TO PUBLIC KNOWLEDGE.

990 for the year ended December 31, 2024

Program accomplishments

  • IN 2024, THE TWO FRONTIERS PROJECT ADVANCED ITS CARBON INITIATIVE, FOCUSED ON DISCOVERING AND CHARACTERIZING NOVEL MICROORGANISMS THAT NATURALLY CAPTURE AND RECYCLE CARBON. THE TEAM CONDUCTED FIELDWORK IN JAPAN AND PAPUA NEW GUINEA, ISOLATING AND ANALYZING CYANOBACTERIA SUCH AS THE NEWLY IDENTIFIED CHONKUS LINEAGE CAPABLE OF ENHANCED CARBON FIXATION IN EXTREME ENVIRONMENTS. THESE DISCOVERIES SUPPORTED THE DEVELOPMENT OF A NEW LABORATORY AND DATA PLATFORM FOR ASSESSING MICROBIAL CARBON-CAPTURE POTENTIAL. THE ORGANIZATION ALSO LAUNCHED COMPLEMENTARY CITIZEN SCIENCE PROGRAMS, INCLUDING "IN YOUR HOME AND "IN THE WILD", WHICH TRAINED MEMBERS OF THE PUBLIC TO COLLECT ENVIRONMENTAL SAMPLES FOR ANALYSIS. EDUCATIONAL WORKSHOPS ACCOMPANYING THESE PROGRAMS INTRODUCED PARTICIPANTS TO MICROBIOLOGY, CULTURING TECHNIQUES, AND THE ROLE OF MICROORGANISMS IN MITIGATING POLLUTION AND MAINTAINING ECOSYSTEM BALANCE.
    Expenses $114,094 · grants $50,000
  • THROUGH ITS CROPS INITIATIVE, THE TWO FRONTIERS PROJECT BEGAN BUILDING A RESEARCH PLATFORM TO IDENTIFY AND CULTURE MICROORGANISMS THAT ENHANCE AGRICULTURAL PRODUCTIVITY, WATER RETENTION, AND PLANT RESILIENCE UNDER DROUGHT AND NUTRIENT STRESS. IN 2024, THE ORGANIZATION CONDUCTED AN EXPEDITION TO THE MOJAVE DESERT TO STUDY NATURALLY STRESS-ADAPTED MICROBIAL COMMUNITIES ASSOCIATED WITH DESERT FLORA AND SOIL CRUSTS. THESE FINDINGS INFORMED THE DESIGN OF FUTURE EXPERIMENTS FOCUSED ON BIOLOGICAL SUPPORT SYSTEMS FOR REGENERATIVE AGRICULTURE. CONCURRENTLY, 2FP EXPANDED ITS LABORATORY INFRASTRUCTURE FOR MICROBIAL ISOLATION, CULTURE, AND FUNCTIONAL TESTING, LAYING THE GROUNDWORK FOR A SCALABLE DATABASE AND OPEN TOOLKIT FOR ENVIRONMENTAL AND CROP-ASSOCIATED MICROBIOME RESEARCH.
    Expenses $106,296
  • THE CORAL INITIATIVE FOCUSED ON THE DEVELOPMENT OF OPEN-SOURCE BIOINFORMATIC SOFTWARE AND WORKFLOWS FOR ANALYZING CORAL-ASSOCIATED MICROBIAL COMMUNITIES. THE ORGANIZATION ADVANCED TWO FLAGSHIP TOOLS MAGUS (HTTPS://GITHUB.COM/TWO-FRONTIERS-PROJECT/2FP_MAGUS, FOR METAGENOMIC ASSEMBLY AND BINNING) AND XTREE (HTTPS://GITHUB.COM/TWO-FRONTIERS-PROJECT/2FP-XTREE, FOR TAXONOMIC AND FUNCTIONAL ALIGNMENT)WHICH TOGETHER ENABLE HIGH-RESOLUTION PROFILING OF CORAL METAGENOMES. IN PARALLEL, 2FP ESTABLISHED STANDARDIZED LABORATORY PROTOCOLS FOR ISOLATING AND CULTURING BENEFICIAL MICROORGANISMS FROM CORAL ECOSYSTEMS. THESE EFFORTS FORM THE FOUNDATION FOR ONGOING RESEARCH INTO MICROBIAL PROCESSES THAT INFLUENCE CORAL HEALTH, RESILIENCE, AND POLLUTANT BREAKDOWN. ALL METHODS, CODE, AND REFERENCE DATA GENERATED THROUGH THIS PROGRAM WERE MADE FREELY AVAILABLE TO THE SCIENTIFIC COMMUNITY, SUPPORTING GLOBAL EFFORTS TO SAFEGUARD REEF ECOSYSTEMS AND COASTAL WATER QUALITY.
    Expenses $7,183
  • BRAND
    Expenses $4,844
  • COMPUTE INFRASTRUCTURE
    Expenses $159

What it must file and when

IRS records say it files Form 990 or 990-EZ. Based on its last return; a smaller organization may file the 990-N. Its accounting year ends in December, so each return is due on the 15th day of the 5th month after that.

Tax yearYear endedDueIRS public records
2025December 31, 2025May 15, 2026Not on file
2024December 31, 2024May 15, 2025990 on file
2023December 31, 2023May 15, 2024Check at the IRS

IRS record

EIN
99-2011215
Tax-exempt status
501(c)(3): Charitable, religious, educational or scientific organization
Foundation status
Publicly supported organization (170(b)(1)(A)(vi))
Donations deductible (Pub 78)
Public charity, 50% deduction limit
Exempt since
2024
Accounting year ends
December
Files
Form 990 or 990-EZ
Assets (IRS master file)
$285,745
Income (IRS master file)
$538,377

Filing history

FormYear endedRevenueExpensesAssetsReturn
990December 2024$538,377$255,772$285,745XML

990-N: the IRS bulk file holds only the latest e-Postcard per organization; earlier years come from archived copies of that file since 2020 and can have gaps (the IRS e-Postcard search lists every one). 990, 990-EZ, 990-PF, 990-T: every e-filed return in the IRS e-file index since 2019; XML for the latest three processing years, amounts from the IRS Statistics of Income extracts before that. PDF copies and determination letters at the IRS (search EIN 99-2011215).

Questions about TWO FRONTIERS PROJECT INC

What is the EIN of TWO FRONTIERS PROJECT INC?
The employer identification number (EIN) of TWO FRONTIERS PROJECT INC is 99-2011215.
Is TWO FRONTIERS PROJECT INC tax-exempt?
Yes. The IRS lists it as tax-exempt under section 501(c)(3) since 2024.
Are donations to TWO FRONTIERS PROJECT INC tax-deductible?
Yes. It is listed in IRS Publication 78 (Public charity, 50% deduction limit).
Which annual return does TWO FRONTIERS PROJECT INC file?
Form 990 or 990-EZ. Based on its last return; a smaller organization may file the 990-N.
When is the next return of TWO FRONTIERS PROJECT INC due?
The return for tax year 2025 (the year ending December 31, 2025) is due May 15, 2026. Form 8868 extends it by six months; the 990-N cannot be extended.
When did TWO FRONTIERS PROJECT INC last file with the IRS?
The latest return in the IRS public files is the 990 for the year ended December 2024.
Who is the principal officer of TWO FRONTIERS PROJECT INC?
The 990 for the year ended December 31, 2024 names BRADEN TIERNEY.
Who are the officers and directors of TWO FRONTIERS PROJECT INC?
The 990 for the year ended December 31, 2024 lists 4 people; 1 received compensation from the organization, the highest $4,263 (KRISTA RYON, TREASURER AND DIRECTOR OF OPERATIONS).
How much revenue does TWO FRONTIERS PROJECT INC have?
On its 990 for the year ended December 31, 2024 it reported revenue of $538,377, expenses of $255,772 and total assets of $285,745 at year end.

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