2025-10-29T10:34:09-05:00 2024-12-31 390974031 WEGNER CPAS LLP 419 N LEE ST ALEXANDRIA VA 223142301 990 2024-03-19 992011215 THE TWO FRONTIERS PROJECT INC TWOF 7816262626 3336 DUDLEY WAY FORT COLLINS CO 80526 BRADEN TIERNEY EXECUTIVE DIRECTOR 7816262626 2025-10-28 1 1 ADAM KUCZYNSKI CPA P01560606 7035190990 2025-10-28 2024 2025-03-06 01:10:19Z X BRADEN TIERNEY 3336 DUDLEY WAY FORT COLLINS CO 80526 538377 0 X WWW.TWOFRONTIERS.ORG X 2024 DE TO EXPLORE, UNDERSTAND, AND HARNESS MICROBIAL LIFE-FROM SEA TO SPACE. 4 2 1 4 0 0 538219 0 0 158 538377 50000 0 12915 0 1623 192857 255772 282605 285745 3390 282355 X THE PRINCIPAL PURPOSE OF TWO FRONTIERS IS TO ADVANCE HUMANITY'S UNDERSTANDING OF LIFE'S ADAPTATION TO EXTREME ENVIRONMENTS THROUGH SCIENTIFIC RESEARCH AND EDUCATION. THE ORGANIZATION PLANS AND LEADS SCIENTIFIC EXPEDITIONS TO REMOTE OCEANIC, TERRESTRIAL, AND OTHER HIGH-STRESS ENVIRONMENTS TO STUDY HOW MICROORGANISMS SURVIVE AND INFLUENCE NATURAL SYSTEMS.USING MODERN SEQUENCING AND MICROBIOLOGY TOOLS, TWO FRONTIERS COLLECTS, ANALYZES, AND SHARES DATA TO SUPPORT ENVIRONMENTAL RECOVERY AND CONTAMINATION MITIGATION. ITS OPEN-ACCESS DATABASES, SOFTWARE, AND PUBLICATIONS ARE FREELY AVAILABLE TO RESEARCHERS FOR NON-COMMERCIAL PURPOSES. THE ORGANIZATION MAY ALSO FUND ACADEMIC OR NONPROFIT PARTNERS TO CONDUCT RELATED SCIENTIFIC STUDIES THAT CONTRIBUTE TO PUBLIC KNOWLEDGE. 0 0 114094 50000 0 IN 2024, THE TWO FRONTIERS PROJECT ADVANCED ITS CARBON INITIATIVE, FOCUSED ON DISCOVERING AND CHARACTERIZING NOVEL MICROORGANISMS THAT NATURALLY CAPTURE AND RECYCLE CARBON. THE TEAM CONDUCTED FIELDWORK IN JAPAN AND PAPUA NEW GUINEA, ISOLATING AND ANALYZING CYANOBACTERIA SUCH AS THE NEWLY IDENTIFIED CHONKUS LINEAGE CAPABLE OF ENHANCED CARBON FIXATION IN EXTREME ENVIRONMENTS. THESE DISCOVERIES SUPPORTED THE DEVELOPMENT OF A NEW LABORATORY AND DATA PLATFORM FOR ASSESSING MICROBIAL CARBON-CAPTURE POTENTIAL. THE ORGANIZATION ALSO LAUNCHED COMPLEMENTARY CITIZEN SCIENCE PROGRAMS, INCLUDING "IN YOUR HOME AND "IN THE WILD", WHICH TRAINED MEMBERS OF THE PUBLIC TO COLLECT ENVIRONMENTAL SAMPLES FOR ANALYSIS. EDUCATIONAL WORKSHOPS ACCOMPANYING THESE PROGRAMS INTRODUCED PARTICIPANTS TO MICROBIOLOGY, CULTURING TECHNIQUES, AND THE ROLE OF MICROORGANISMS IN MITIGATING POLLUTION AND MAINTAINING ECOSYSTEM BALANCE. 106296 0 0 THROUGH ITS CROPS INITIATIVE, THE TWO FRONTIERS PROJECT BEGAN BUILDING A RESEARCH PLATFORM TO IDENTIFY AND CULTURE MICROORGANISMS THAT ENHANCE AGRICULTURAL PRODUCTIVITY, WATER RETENTION, AND PLANT RESILIENCE UNDER DROUGHT AND NUTRIENT STRESS. IN 2024, THE ORGANIZATION CONDUCTED AN EXPEDITION TO THE MOJAVE DESERT TO STUDY NATURALLY STRESS-ADAPTED MICROBIAL COMMUNITIES ASSOCIATED WITH DESERT FLORA AND SOIL CRUSTS. THESE FINDINGS INFORMED THE DESIGN OF FUTURE EXPERIMENTS FOCUSED ON BIOLOGICAL SUPPORT SYSTEMS FOR REGENERATIVE AGRICULTURE. CONCURRENTLY, 2FP EXPANDED ITS LABORATORY INFRASTRUCTURE FOR MICROBIAL ISOLATION, CULTURE, AND FUNCTIONAL TESTING, LAYING THE GROUNDWORK FOR A SCALABLE DATABASE AND OPEN TOOLKIT FOR ENVIRONMENTAL AND CROP-ASSOCIATED MICROBIOME RESEARCH. 7183 0 0 THE CORAL INITIATIVE FOCUSED ON THE DEVELOPMENT OF OPEN-SOURCE BIOINFORMATIC SOFTWARE AND WORKFLOWS FOR ANALYZING CORAL-ASSOCIATED MICROBIAL COMMUNITIES. THE ORGANIZATION ADVANCED TWO FLAGSHIP TOOLS MAGUS (HTTPS://GITHUB.COM/TWO-FRONTIERS-PROJECT/2FP_MAGUS, FOR METAGENOMIC ASSEMBLY AND BINNING) AND XTREE (HTTPS://GITHUB.COM/TWO-FRONTIERS-PROJECT/2FP-XTREE, FOR TAXONOMIC AND FUNCTIONAL ALIGNMENT)WHICH TOGETHER ENABLE HIGH-RESOLUTION PROFILING OF CORAL METAGENOMES. IN PARALLEL, 2FP ESTABLISHED STANDARDIZED LABORATORY PROTOCOLS FOR ISOLATING AND CULTURING BENEFICIAL MICROORGANISMS FROM CORAL ECOSYSTEMS. THESE EFFORTS FORM THE FOUNDATION FOR ONGOING RESEARCH INTO MICROBIAL PROCESSES THAT INFLUENCE CORAL HEALTH, RESILIENCE, AND POLLUTANT BREAKDOWN. ALL METHODS, CODE, AND REFERENCE DATA GENERATED THROUGH THIS PROGRAM WERE MADE FREELY AVAILABLE TO THE SCIENTIFIC COMMUNITY, SUPPORTING GLOBAL EFFORTS TO SAFEGUARD REEF ECOSYSTEMS AND COASTAL WATER QUALITY. 4844 0 0 BRAND 159 0 0 COMPUTE INFRASTRUCTURE 5003 0 0 232576 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 X 4 2 0 0 0 0 0 0 0 1 1 0 0 1 1 1 1 0 0 0 0 0 X MISSION FIRST OPERATIONS 2699989730 14700 DRIFTWATER DR WINTER GARDEN FL 34787 BRADEN TIERNEY PRESIDENT AND EXECUTIVE DIRECTOR 40.00 X X 0 0 1510 KRISTA RYON TREASURER AND DIRECTOR OF OPERATIONS 40.00 X X 4263 0 1507 JAMES HENRIKSEN SECRETARY AND DIRECTOR OF RESEARCH AND DEVELOPMENT 20.00 X X 0 0 0 ERIC WARD DIRECTOR 2.00 X 0 0 0 4263 0 3017 0 0 0 0 0 538219 123464 538219 158 158 158 538377 0 0 158 X 50000 50000 12174 8146 2509 1519 741 496 153 92 550 550 7340 7340 28203 28173 20 10 352 352 4189 773 3414 2 2294 1954 340 33596 28898 4698 3209 3209 2300 2300 TOOLS AND SUPPLIES 109807 109558 249 DUES AND SUBSCRIPTIONS 1017 1017 255772 232576 21573 1623 0 265045 23000 2300 0 20700 0 285745 0 3390 0 3390 X 0 282355 0 282355 0 285745 X 282605 -250 X 0 0 0 X 538219 538219 538219 538219 201936 336283 538219 158 X SCHEDULE A, PART II: THE 2024 COLUMN IS FOR THE SHORT YEAR 3/19/24-12/31/24. RESTRICTED RESTRICTED RESTRICTED RESTRICTED RESTRICTED RESTRICTED RESTRICTED RESTRICTED 23000 2300 20700 20700 0 COLORADO STATE UNIVERSITY 1060 WARNER STREET FORT COLLINS CO 80523 846000545 GOV 50000 0 SUPPORT THE ONGOING EFFORTS OF NREL'S CULTUROMICS TEAM 1 0 PART I, LINE 2: THE ORGANIZATION DOES NOT HAVE ANY FORMAL PROCEDURES IN PLACE TO MONITOR THE USE OF GRANT FUNDS. X FREEZERS AND LAB EQUIPMENT 1 123464 COST 0 0 0 0 PART I, COLUMN (B): THE NUMBER OF CONTRIBUTIONS LISTED ABOVE IS THE NUMBER OF CONTRIBUTIONS MADE. ALL FREEZERS AND LAB EQUIPMENT WERE DONATED FROM ONE CONTRIBUTOR ON MULTIPLE DATES. FORM 990, PART VI, SECTION B, LINE 11B THE FORM 990 WAS PROVIDED TO THE ORGANIZATION'S GOVERNING BODY BEFORE IT WAS FILED WITH THE IRS. FORM 990, PART VI, SECTION B, LINE 12C POTENTIAL CONFLICTS OF INTEREST ARE DISCUSSED IN BOARD MEETINGS AND ANY MEMBERS WITH AN ACTIVE CONFLICT OF INTEREST ARE RECUSED DURING RELEVANT SECTIONS OF THE BOARD MEETINGS. FORM 990, PART VI, SECTION C, LINE 19 THE ORGANIZATION MAKES ITS GOVERNING DOCUMENTS, CONFLICT OF INTEREST POLICY, AND FINANCIAL STATEMENTS AVAILABLE TO THE PUBLIC UPON REQUEST. FORM 990, PART IX, LINE 11G OTHER PROFESSIONAL FEES: PROGRAM SERVICE EXPENSES 28,111. MANAGEMENT AND GENERAL EXPENSES 0. FUNDRAISING EXPENSES 0. TOTAL EXPENSES 28,111. PAYROLL PROCESSING FEES: PROGRAM SERVICE EXPENSES 62. MANAGEMENT AND GENERAL EXPENSES 20. FUNDRAISING EXPENSES 10. TOTAL EXPENSES 92. FORM 990, PART XI, LINE 9: FOREIGN CURRENCY EXCHANGE GAIN/LOSS -250.